Explore the principles and techniques of fluorescent stains, their role in biological imaging, and key considerations for accurate signal interpretation.

Discover expert tips, protocols & high-quality reagents for immunofluorescence staining. Enhance your imaging for clear, reproducible results.

To perform immunofluorescence staining, a fluorophore must be conjugated ("tagged") to an antibody. Staining procedures can be applied to both retained intracellular expressed antibodies, or to cell …

2 days ago · Immunofluorescence encompasses a number of related techniques that have evolved over time. All of these techniques leverage the specific recognition of biological targets by antibodies and …

May 3, 2023 · Fluorescent staining is an advanced molecular biology technique used in pharmaceutical research to allow scientists to visualize specific cellular structures and molecules.

IF STAINING CONTROLS In order to understand and interpret the IF images obtained, different control stainings can be performed that help to reveal unspecific staining.

Here, we describe preparation of specimens preserved in different types of media and step-by-step methods for both direct and indirect immunofluorescence staining.

Immunofluorescence (IF) staining labels a specific target antigen with a fluorescent dye such as fluorescein isothiocyanate or cyanine. The fluorescent stain visualized under a fluorescent …

LipidSpot™ fluorescent stains show minimal background staining of cellular membranes or other organelles, unlike dyes like Nile Red. Available with green or deep-red/far-red fluorescence.

Learn more about immunofluorescence and immunohistochemistry (IHC) including the differences between fluorescence and chromogenic detection and indirect staining methods.