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    https://en.wikipedia.org › wiki › Gene_knockout

    Gene knockout - Wikipedia

    • Gene knockouts (also known as gene deletion or gene inactivation) are a widely used genetic engineering technique that involves the targeted removal or inactivation of a specific gene within an organism's genome. This can be done through a variety of methods, including homologous recombination, CRISPR-Cas9, and TALENs. One of the main advantage… See more

    Methods

    Knockouts are accomplished through a variety of techniques. Originally, naturally occurring mutations were … See more

    Gene knockout by mutation

    Gene knockout by mutation is commonly carried out in bacteria. An early instance of the use of this technique in Escherichia coli was published in 1989 by Hamilton, et al. In this experiment, two sequential recombinati… See more

    Gene silencing

    For gene knockout investigations, RNA interference (RNAi), a more recent method, also known as gene silencing, has gained popularity. In RNA interference (RNAi), messenger RNA for a particular gene is inactivated … See more

    Types

    A conditional gene knockout allows gene deletion in a tissue in a tissue specific manner. This is required in place of a gene knockout if the null mutation would lead to embryonic death, or a specific tissue or cell type is of s… See more

    Use

    Knockouts are primarily used to understand the role of a specific gene or DNA region by comparing the knockout organism to a wildtype with a similar genetic background.
    Knockout organisms are also used as screening tools … See more

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    Table of Contents
    Methods
    Gene knockout by mutation
    Gene silencing
    Types
    Use
    See also
    External links
     
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